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Image Search Results
Journal: Experimental and Therapeutic Medicine
Article Title: Involvement of neurotrophic signaling in doxorubicin-induced cardiotoxicity
doi: 10.3892/etm.2019.8276
Figure Lengend Snippet: Effects of DOX on the expression of BDNF and NGF in the serum and heart. (A) BDNF concentration in the serum. (B) BDNF mRNA levels in the heart. (C) BDNF protein expression levels in the heart. (D) NGF concentration in the serum. (E) NGF mRNA levels in the heart. (F) NGF protein levels in the heart. Data are expressed as the mean ± standard deviation (n=8–9). *P<0.05 and **P<0.01 compared with Con. NGF, nerve growth factor; BDNF, brain derived neurotrophic factor; DOX, doxorubicin; Con, control.
Article Snippet: Determination of
Techniques: Expressing, Concentration Assay, Standard Deviation, Derivative Assay
Journal: Neural Regeneration Research
Article Title: Enteric glia mediate neuronal outgrowth through release of neurotrophic factors
doi: 10.3969/j.issn.1673-5374.2012.028.001
Figure Lengend Snippet: Enzyme-linked immunosorbent assay (ELISA) analysis of neurotrophic factor release of enteric glia (EG) cells. EG cells were maintained in a standard culture medium containing 10% fetal bovine serum for 24 hours. Medium was changed to one with 2% fetal bovine serum, cells were cultured continually for another 48 hours, and then medium was removed and examined for neurotrophic factor concentration by ELISA. EG cells released NGF, BDNF and GDNF under controlled conditions, with their respective concentrations reaching 250, 96.4 and 53.8 pg/mL respectively. All bars represent mean ± SEM of five independent experiments. NGF: Nerve growth factor; BDNF: brain-derived neurotrophic factor; GDNF: glial cell line-derived neurotrophic factor.
Article Snippet: Subsequently, the cells were centrifuged and the growth factors were measured in the supernatant using commercially available ELISA kits for NGF, BDNF, NT-3 and
Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Concentration Assay, Derivative Assay